RAG1-DNA Binding in V(D)J Recombination
نویسندگان
چکیده
منابع مشابه
VDJ Recombination
Artemis is the newest player in VDJ recombination and double strand break repair. First identified in radiation-sensitive and immune-deficient patients, it was recently shown to interact with DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and have nuclease activity, becoming the most popular candidate for the opening of hairpin coding ends. Reports presented in this issue (1) and in ...
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ment, producing two coding ends and two signal ends. Howard Hughes Medical Institute Subsequent joiningof thecoding ends, forming a coding Baylor College of Medicine joint, assembles a rearranged TCR or Ig gene segment; Houston, Texas 77030 the signal ends also join, forming a signal joint. †Department of Molecular Biology and Genetics Several features of VDJ recombination are reminisHoward Hug...
متن کاملThe RAG1 homeodomain recruits HMG1 and HMG2 to facilitate recombination signal sequence binding and to enhance the intrinsic DNA-bending activity of RAG1-RAG2.
V(D)J recombination is initiated by the specific binding of the RAG1-RAG2 (RAG1/2) complex to the heptamer-nonamer recombination signal sequences (RSS). Several steps of the V(D)J recombination reaction can be reconstituted in vitro with only RAG1/2 plus the high-mobility-group protein HMG1 or HMG2. Here we show that the RAG1 homeodomain directly interacts with both HMG boxes of HMG1 and HMG2 (...
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Currently five polymerases have been identified in Escherichia coli, at least eight in Saccharo‐ myces cerevisiae, nine in Schizosaccharomyces pombe, and fourteen in humans [1-4]. Based on the primary structure of the catalytic subunits, DNA polymerases have been classified into different families. Eukaryotic organisms have four families: A family (Polγ, Polθ and Polν), B family (Polα, Polδ, Po...
متن کاملPromoters, enhancers, and transcription target RAG1 binding during V(D)J recombination
V(D)J recombination assembles antigen receptor genes in a well-defined order during lymphocyte development. This sequential process has long been understood in the context of the accessibility model, which states that V(D)J recombination is regulated by controlling the ability of the recombination machinery to gain access to its chromosomal substrates. Indeed, many features of "open" chromatin ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2003
ISSN: 0021-9258
DOI: 10.1074/jbc.m209758200